![]() We also evaluate efficiency of a subset of the algorithms implemented. ![]() We validate the implementation using synthetic datasets and actual experimental imaging data derived from publicly available resources. The main components include routines for reconstruction with or without using external reference and solutions for typical issues encountered during the reconstruction process, such as propagation of the registration errors due to distorted sections. ![]() Here we propose the Possum volumetric reconstruction framework which provides a selection of 2D to 3D image reconstruction routines allowing one to build workflows tailored to one’s specific requirements. Currently, there are no tools for performing such a task in a streamlined way. However, to compare or combine them with results from three dimensional imaging methods, reconstruction into a volumetric form is required. ![]() Techniques based on imaging serial sections of brain tissue provide insight into brain structure and function. ![]()
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